Microbial Rhodopsins >> Bacteriorhodopsin
NAME:Bacteriorhodopsin
PDB IDs: 1AP9, 1BRX, 1C3W, 1C8R, 1C8S, 1CWQ, 1E0P, 1F4Z, 1F50, 1KG8, 1KG9, 1KGB, 1M0K, 1M0L, 1M0M, 1O0A, 1P8H, 1P8I, 1P8U, 1QHJ, 1QKO, 1QKP, 1VJM, 2I1X, 2I20, 2I21, 2NTU, 2NTW, 2WJK, 2WJL, 3MBV, 3NS0, 3NSB, 4FPD
SOURCE ORGANISM: Halobacterium salinarum
SEQUENCE: UniProt P02945>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP 200 nL | Ppt solution 2 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid: 100 %w/w Monoolein | Protein: 10 - 20 mg/mL bR | Salt: 2 - 3 M Na/K phosphate pH 5.6 |
| Buffer: 25 mM Na/K phosphate pH 5.6 | ||
| Detergent: 1.5 %w/v OG |
Temperature (°C): 20
Crystal growth time (d): 7 - 30 Crystal size (µm): 80 x 80 x 15
Comments:
Reference: H. Luecke, B. Schobert, H. T. Richter, J. P. Cartailler and J. K. Lanyi (1999) Structure of bacteriorhodopsin at 1.55 A resolution J Mol Biol 291: 899-911 >>
SPACE GROUP: P 63 UNIT CELL: 60.63 60.63 108.16 90.0 90.0 120.0
CRYSTAL PACKING:
