Microbial rhodopsins >> Halorhodopsin
NAME:Halorhodopsin
PDB IDs: 1E12
SOURCE ORGANISM: Halobacterium salinarum
SEQUENCE: UniProt P16102>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP | Ppt solution |
| Lipid 58-62 %w/w | Protein Solution 38-42 %w/w | |
| Lipid: Monoolein | Protein: 8 - 10 mg/mL hR | Salt: 4 M K chloride |
| Buffer: 50 mM Tris pH 6.5-7.4 | ||
| Detergent: 1 %w/v OG |
Temperature (°C): 22 Crystal growth time (d): 14 Crystal size (µm): 100 x 100 x 20
Comments:
Reference: Kolbe, M., Besir, H., Essen, L.-O., Oesterhelt, D. (2000) Structure of the light-driven chloride pump halorhodopsin at 1.8 Å resolution. Science 288: 1390-1396 >>
SPACE GROUP: P 63 2 2 UNIT CELL: 67.3 67.3 209.2 90.0 90.0 120.0
CRYSTAL PACKING:
