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Microbial rhodopsins >> Sensory rhodopsin II

NAME:Sensory rhodopsin II

PDB IDs: 1H68, 1JGJ, 1GU8, 1GUE, 3QAP, 3QDC

SOURCE ORGANISM: Natronomonas pharaonis

SEQUENCE: UniProt P42196>>

ORIENTATION IN MEMBRANE: OPM >>

CRYSTALLIZATION CONDITIONS:

LCP 0.2 μL Ppt solution 5 μL
Lipid 60 %w/w Protein Solution 40 %w/w
Lipid: Monoolein Protein: 11.2 mg/mL sR II Salt: 1.5 M Na chloride
Detergent: 0.01 %w/v DDM Buffer: 75 mM Na Acetate pH 4.6
Additive: Polar lipids from Halobacterium salinarum

Temperature (°C): 20           Crystal growth time (d): 14           Crystal size (µm): 300

Comments:

Reference: Royant, A., Nollert, P., Edman, K., Neutze, R., Landau, E.M., Pebay-Peyroula, E., Navarro, J. (2001) X-ray structure of sensory rhodopsin II at 2.1 Å resolution. Proc Natl Acad Sci USA 98: 10131-10136 >>

SPACE GROUP: C 2 2 21             UNIT CELL: 84.83 128.74 50.74 90.0 90.0 90.0

CRYSTAL PACKING:

CONTACT US: USC | Cherezov Lab | cherezov@usc.edu