Microbial rhodopsins >> Sensory rhodopsin II
NAME:Sensory rhodopsin II
PDB IDs: 1H68, 1JGJ, 1GU8, 1GUE, 3QAP, 3QDC
SOURCE ORGANISM: Natronomonas pharaonis
SEQUENCE: UniProt P42196>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP 0.2 μL | Ppt solution 5 μL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid: Monoolein | Protein: 11.2 mg/mL sR II | Salt: 1.5 M Na chloride |
| Detergent: 0.01 %w/v DDM | Buffer: 75 mM Na Acetate pH 4.6 | |
| Additive: Polar lipids from Halobacterium salinarum |
Temperature (°C): 20 Crystal growth time (d): 14 Crystal size (µm): 300
Comments:
Reference: Royant, A., Nollert, P., Edman, K., Neutze, R., Landau, E.M., Pebay-Peyroula, E., Navarro, J. (2001) X-ray structure of sensory rhodopsin II at 2.1 Å resolution. Proc Natl Acad Sci USA 98: 10131-10136 >>
SPACE GROUP: C 2 2 21 UNIT CELL: 84.83 128.74 50.74 90.0 90.0 90.0
CRYSTAL PACKING:
