Microbial rhodopsins >> Bacteriorhodopsin
NAME:Bacteriorhodopsin
PDB IDs: 1JV6, 1JV7, 1MGY, 1S8J, 1S8L
SOURCE ORGANISM: Halobacterium salinarum
SEQUENCE: UniProt P02945>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP | Ppt solution |
| Lipid 65 %w/w | Protein Solution 35 %w/w | |
| Lipid: Monoolein | Protein: 20 - 30 mg/mL bR | |
| Additive: Retinal | Buffer: 25 mM Na/K phosphate pH 5.6 | |
| Detergent: 1.5 %w/v OG |
Temperature (°C): 20 Crystal growth time (d): < 30 Crystal size (µm): 200 x 30 x 5
Comments:
Reference: Rouhani, S., Cartailler, J.P., Facciotti, M.T., Walian, P. Needleman, R., Lanyi, J.K., Glaeser, R.M., Luecke, H. (2001) Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediate J Mol Biol 313: 615-628 >>
SPACE GROUP: C 2 2 21 UNIT CELL: 51.8 121.3 85.7 90.0 90.0 90.0
CRYSTAL PACKING:
