Structures from LCP >> Reaction Center
NAME:Photosynthetic Reaction Center
PDB IDs: 1OGV, 2GNU, 2BNP, 2BNS
SOURCE ORGANISM: Rhodobacter sphaeroides
SEQUENCE: UniProt P0C0Y7>> , UniProt P0C0Y8>> , UniProt P0C0Y9>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP, 1 part | Ppt solution, 4 parts |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid: Monoolein | Protein: 25 mg/mL RC | Buffer: 1 M Hepes pH 7.5 |
| Detergent: 0.1 %w/v LDAO | Precipitant: 18 %w/v Jeffamine M-600 | |
| Buffer: 10 mM Tris pH 8.0 | Salt: 0.7 M Ammonium sulfate | |
| Additive: 0.1 mM EDTA |
Temperature (°C): Room Temperature
Crystal growth time (d): 3 Crystal size (µm): 50 x 50 x 20
Comments:
Reference: Katona, G., Andreasson, U., Landau, E.M., Andreasson, L.-E., and Neutze, R.(2003) Lipidic cubic phase crystal structure of the photosynthetic reactioin centre from Rhodobacter sphaeroides at 2.35 A resolution. J Mol Biol 321: 681-692 >>
SPACE GROUP: C 2 2 21 UNIT CELL: 103.1 116.51 56.19 90.0 90.0 90.0
CRYSTAL PACKING:
