Microbial rhodopsins >> Sensory rhodopsin
NAME:Sensory rhodopsin
PDB IDs: 1XIO
SOURCE ORGANISM: Nostoc sp. pcc 7120
SEQUENCE: UniProt Q8YSC4 >>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP | Ppt solution |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid: Monoolein | Protein: 9 mg/mL sR | Buffer: 100 mM tri-sodium citrate pH 5.6 |
| Detergent: 0.01 %w/v DDM | Salt: 100 mM Mg chloride | |
| Additive: 4 %v/v 2-methyl-2,4-pentanediol |
Temperature (°C): 20 Crystal growth time (d): 3-5 Crystal size (µm): 20 x 50 x 50
Comments:
Reference: Vogeley, L., Sineshchekov, O.A., Trivedi, V.D., Sasaki, J., Spudich, J.L., and Luecke, H. (2004) Anabaena sensory rhodopsin: a photochromic color sensor at 2.0 A. Science 306: 1390-1393 >>
SPACE GROUP: C 2 2 21 UNIT CELL: 103.1 116.51 56.19 90.0 90.0 90.0
CRYSTAL PACKING:
