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Structures from LCP >> Light harvesting complex 2

NAME:Light harvesting complex 2

PDB IDs: 2FKW

SOURCE ORGANISM: Rhodoblastus acidophilus

SEQUENCE: UniProt P26789>> UniProt P26790>>

ORIENTATION IN MEMBRANE: OPM >>

CRYSTALLIZATION CONDITIONS:

LCP 200 nL Ppt solution 2 µL
Lipid 60 %w/w Protein Solution 40 %w/w
Lipid: 100 %w/w Monoolein Protein: 10 mg/mL LH2B Buffer: 100 mM Tris pH 8.0
Buffer: 20 mM Tris pH 8.0 Precipitant: 20 %v/v PPO
Detergent: 0.05 %w/v LDAO Salt: 0.1 M K formate

Temperature (°C): 20

Crystal growth time (d): 3 - 7           Crystal size (µm): 200 x 30 x 10

Comments:

Reference: V. Cherezov, J. Clogston, M.Z. Papiz, and M. Caffrey. 2006. Room to move: crystallizing membrane proteins in swollen lipidic mesophases. J Mol Biol 357: 1605-1618 >>

SPACE GROUP: P 21 21 21           UNIT CELL: 81.44 126.38 129.71 90.0 90.0 90.0

CRYSTAL PACKING:

CONTACT US: USC | Cherezov Lab | cherezov@usc.edu