NAME:Light harvesting complex 2
PDB IDs: 2FKW
SOURCE ORGANISM: Rhodoblastus acidophilus
SEQUENCE: UniProt P26789>> UniProt P26790>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
LCP 200 nL | Ppt solution 2 µL |
Lipid 60 %w/w | Protein Solution 40 %w/w | |
Lipid: 100 %w/w Monoolein | Protein: 10 mg/mL LH2B | Buffer: 100 mM Tris pH 8.0 |
Buffer: 20 mM Tris pH 8.0 | Precipitant: 20 %v/v PPO | |
Detergent: 0.05 %w/v LDAO | Salt: 0.1 M K formate |
Temperature (°C): 20
Crystal growth time (d): 3 - 7           Crystal size (µm): 200 x 30 x 10
Comments:
Reference: V. Cherezov, J. Clogston, M.Z. Papiz, and M. Caffrey. 2006. Room to move: crystallizing membrane proteins in swollen lipidic mesophases. J Mol Biol 357: 1605-1618 >>
SPACE GROUP: P 21 21 21           UNIT CELL: 81.44 126.38 129.71 90.0 90.0 90.0
CRYSTAL PACKING:
CONTACT US: USC | Cherezov Lab | cherezov@usc.edu