Vitamin B₁₂ transporter BtuB

NAME:Outer membrane vitamin B₁₂ transporter BtuB

PDB IDs: 2GUF

SOURCE ORGANISM: E. coli

ORIENTATION IN MEMBRANE: OPM >>

CRYSTALLIZATION CONDITIONS:

LCP 200 nL

Ppt solution 3 µL

Lipid 60 %w/w	Protein Solution 40 %w/w
Lipid: 100 %w/w Monoolein	Protein: 15 mg/mL BtuB	Buffer: 100 mM MES pH 6.5
	Buffer: 10 mM Tris pH 8.0	Precipitant: 11 %v/v MPD
	Detergent: 0.1 %w/v LDAO	Salt: 0.15 M NH4 formate

Temperature (°C): 20 Crystal growth time (d): 10 - 14 Crystal size (µm): 100 x 60 x 25

Comments:

Reference: V. Cherezov, E. Yamashita, W. Liu, M. Zhalnina, W. A. Cramer and M. Caffrey. 2006. In meso structure of the cobalamin transporter, BtuB, at 1.95 A resolution. J Mol Biol 364: 716-734 >>

SPACE GROUP: P 21 21 21 UNIT CELL: 72.61 80.88 118.68 90.0 90.0 90.0

CRYSTAL PACKING:

Outer membrane proteins >> Vitamin B12 transporter BtuB

Outer membrane proteins >> Vitamin B₁₂ transporter BtuB