Microbial rhodopsins >> Halorhodopsin
NAME:Halorhodopsin
SOURCE ORGANISM: Halobacterium salinarum
SEQUENCE: UniProt P16102>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP | Ppt solution |
| Lipid 58-62 %w/w | Protein Solution 38-42 %w/w | |
| Lipid: Monoolein | Protein: 9 mg/mL hR | Salt: 3.3 M K chloride |
| Buffer: 25 mM Tris pH 7 | ||
| Detergent: 1 %w/v OG |
Temperature (°C): 22 Crystal growth time (d): Crystal size (µm): 150 x 50 x 15
Comments:
Reference: Gmelin, W., Zeth, K., Efremov, R., Heberle, J., Tittor, J., Oesterhelt, D. (2007) The crystal structure of the L1 intermediate of halorhodopsin at 1.9 angstroms resolution. Photochem Photobiol 83: 369-377 >>
SPACE GROUP: H 3 2 UNIT CELL: 94.47 94.47 157.14 90.0 90.0 120.0
CRYSTAL PACKING:
