Structures from LCP >> β2-Adrenergic Receptor
NAME:β2-Adrenergic Receptor
PDB IDs: 2RH1
SOURCE ORGANISM: Homo sapiens
SEQUENCE: UniProt P07550>>
ORIENTATION IN MEMBRANE: OPM >>
GPCRDB: ADRB2_HUMAN >>
CRYSTALLIZATION CONDITIONS:
| LCP 50 nL | Ppt solution 0.8 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid 1: 90 %w/w Monoolein | Protein: 50 mg/mL β2AR-T4L | Buffer: 100 mM Bis tris propane pH 6.8 |
| Lipid 2: 10 %w/w Cholesterol | Ligand: 10 µM Carazolol | Precipitant: 32 %v/v PEG 400 |
| Buffer: 20 mM Hepes pH 7.5 | Salt: 0.1 M Na sulfate | |
| Detergent 1: 0.1 %w/v DDM | Additive: 5 %v/v 1,4-butanediol | |
| Detergent 2: 0.02%w/v CHS | Salt: 100 mM NaCl |
Temperature (°C): 20
Crystal growth time (d): 3 – 7 Crystal size (µm): 40 x 15 x 5
Reference: V. Cherezov, D. M. Rosenbaum, M. A. Hanson, S. G. Rasmussen, F. S. Thian, T. S. Kobilka, H. J. Choi, P. Kuhn, W. I. Weis, B. K. Kobilka and R. C. Stevens. 2007. High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor. Science 318: 1258-1265 >>
SPACE GROUP: C 2 UNIT CELL: 106.32 169.24 40.15 90.0 105.62 90.0
CRYSTAL PACKING:
