Structures from LCP >> Reaction Center
NAME:Photosynthetic Reaction Center
PDB IDs: 2WJM, 2WJN, 2X5U, 2X5V, 4AC5
SOURCE ORGANISM: Blastochloris viridis
SEQUENCE: UniProt P07173>> , UniProt P06008>> , UniProt P06009>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
MONOOLEIN WAS MIXED WITH WATER IN RATIO OF 60:40 (W/W) UNTIL A VISCOUS NON-BIREFRINGENT LCP WAS FORMED. THEREAFTER, LIPIDIC-SPONGE PHASE INITIATING SOLUTION (20% JEFFAMINE M600, 1M HEPES PH=8.0, 0.7M (NH4)2SO4 AND 2.5% 1,2,3-HEPTANETRIOL) WAS ADDED IN A RATIO OF 1 TO 4 (V/V) AND EQUILIBRATED UNTIL PHASE SEPARATION HAS OCCURRED. THE UPPER LIPIDIC-SPONGE PHASE WAS HARVESTED AND 1UL WAS USED AS A PRECIPITANT SOLUTION TOGETHER WITH 1UL OF 25 MG/ML PROTEIN SOLUTION IN A HANGING-DROP, VAPOUR-DIFFUSION EXPERIMENT. TO IMPROVE CRYSTAL QUALITY 1UL OF ADDITIVE 1M TRI-NA-CITRATE HAS BEEN ADDED TO THE CRYSTALLIZATION DROP.
Temperature (°C): Room Temperature
Crystal growth time (d): 14 Crystal size (µm): 250 x 250 x 150
Comments:
Reference: Wohri, A.B., Wahlgren, W.Y., Malmerberg, E., Johansson, L.C., Neutze, R., Katona, G. 2009. Lipidic-sponge phase crystal structure of a photosynthetic reaction center reveals lipids on the protein surface. Biochemistry 48: 9831-9838 >>
SPACE GROUP: P 21 21 2 UNIT CELL: 84.76 139.43 178.24 90.0 90.0 90.0
CRYSTAL PACKING:
