NAME:Gramicidin D
SOURCE ORGANISM: Brevibacillus brevis
SEQUENCE:
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
LCP 50 nL | Ppt solution 0.8 µL |
Lipid 60 %w/w | Aqueous Solution 40 %w/w | |
Lipid: 80 %w/w Monoolein | Water | Buffer: 100 mM Bicine pH 9.0 |
Peptide: 20% w/w Gramicidin D | Precipitant: 20 %w/v PEG 6000 |
Temperature (°C): 20 Crystal growth time (d): 3-5 Crystal size (µm): 30 x 30 x 30
References: N. Hoefer, D. Aragao and M. Caffrey (2010) Crystallizing transmembrane peptides in lipidic mesophases Biophys J 99: L23-L25 >>
LCP 50 nL | Ppt solution 0.8 µL |
Lipid 50 %w/w | Aqueous Solution 50 %w/w | |
Lipid: 80 %w/w 8.8 MAG | Water | Salt: 200 mM Ammonium sulfate |
Peptide: 20% w/w Gramicidin D | Precipitant: 30 %w/v PEG 8000 |
LCP 50 nL | Ppt solution 0.8 µL |
Lipid 50 %w/w | Aqueous Solution 50 %w/w | |
Lipid: 80 %w/w 7.7 MAG | Water | Buffer: 100 mM Bis-tris pH 5.5 |
Peptide: 20% w/w Gramicidin D | Precipitant: 25 %w/v PEG 3350 | |
Salt: 200 mM Li sulfate |
Temperature (°C): 20 Crystal growth time (d): 3-5 Crystal size (µm): 60 x 60 x 60
References: N. Hoefer, D. Aragao, J. Lyons and M. Caffrey (2011) Membrane protein crystallization in lipidic mesophases. Hosting lipid effects on the crystallization and structure of a transmembrane peptide Cryst Growth Des 11: 1182-1192 >>
SPACE GROUP: P 21             UNIT CELL: 30.50 62.56 30.53     90 99.97 90
CRYSTAL PACKING:
CONTACT US: USC | Cherezov Lab | cherezov@usc.edu