Structures from LCP >> Gramicidin D

NAME:Gramicidin D

PDB IDs: 2XDC, 2Y5M, 2Y6N

SOURCE ORGANISM: Brevibacillus brevis

SEQUENCE:

ORIENTATION IN MEMBRANE: OPM >>

CRYSTALLIZATION CONDITIONS:

LCP 50 nL

Ppt solution 0.8 µL

Lipid 60 %w/w	Aqueous Solution 40 %w/w
Lipid: 80 %w/w Monoolein	Water	Buffer: 100 mM Bicine pH 9.0
Peptide: 20% w/w Gramicidin D		Precipitant: 20 %w/v PEG 6000

Temperature (°C): 20                Crystal growth time (d): 3-5                Crystal size (µm): 30 x 30 x 30

References: N. Hoefer, D. Aragao and M. Caffrey (2010) Crystallizing transmembrane peptides in lipidic mesophases Biophys J 99: L23-L25 >>

LCP 50 nL

Ppt solution 0.8 µL

Lipid 50 %w/w	Aqueous Solution 50 %w/w
Lipid: 80 %w/w 8.8 MAG	Water	Salt: 200 mM Ammonium sulfate
Peptide: 20% w/w Gramicidin D		Precipitant: 30 %w/v PEG 8000

LCP 50 nL

Ppt solution 0.8 µL

Lipid 50 %w/w	Aqueous Solution 50 %w/w
Lipid: 80 %w/w 7.7 MAG	Water	Buffer: 100 mM Bis-tris pH 5.5
Peptide: 20% w/w Gramicidin D		Precipitant: 25 %w/v PEG 3350
		Salt: 200 mM Li sulfate

Temperature (°C): 20                Crystal growth time (d): 3-5                Crystal size (µm): 60 x 60 x 60

References: N. Hoefer, D. Aragao, J. Lyons and M. Caffrey (2011) Membrane protein crystallization in lipidic mesophases. Hosting lipid effects on the crystallization and structure of a transmembrane peptide Cryst Growth Des 11: 1182-1192 >>

SPACE GROUP: P 21             UNIT CELL: 30.50 62.56 30.53     90 99.97 90

CRYSTAL PACKING:

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