Structures from LCP >> caa3 cytochrome c oxidase
NAME:caa3 cytochrome c oxidase
PDB IDs: 2YEV
SOURCE ORGANISM: Thermus thermophilus
SEQUENCE: UniProt P98005>> , UniProt Q5SLI2>> , UniProt Q5SH67>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP 50 nL | Ppt solution 0.8 µL |
| Lipid 50 %w/w | Protein Solution 50 %w/w | |
| Lipid: 100 %w/w MAG 7.7 | Protein: 10 mg/mL caa3 | Buffer: 100 mM Na citrate pH 4.5-5.0 |
| Buffer: 10 mM Tris pH 7.6 | Precipitant: 14-21 %v/v PEG 400 | |
| Detergent: 0.2 %w/v DM | Salt: 0.1 M NaCl | |
| Salt: 0.15 M NaCl | Additive: 0-0.1 M Li sulfate | |
| Additive: 0.2 µM Na ascorbate |
Temperature (°C): 20
Crystal growth time (d): 7-10 Crystal size (µm): 100 x 60 x 5
Comments:
Reference: Lyons, J.A., Aragao, D., Slattery, O., Pisliakov, A.V., Soulimane, T. & Caffrey, M. Structural insights into electron transfer in caa3-type cytochrome oxidase. Nature 487: 514-518 >>
SPACE GROUP: I 1 2 1 UNIT CELL: 127.25 76.03 300.27 90.0 92.21 90.0
CRYSTAL PACKING:
