Structures from LCP >> β2-Adrenergic Receptor
NAME:β2-Adrenergic Receptor
PDB IDs: 3D4S, 3NY8, 3NY9, 3NYA
SOURCE ORGANISM: Homo sapiens
SEQUENCE: UniProt P07550>>
ORIENTATION IN MEMBRANE: OPM >>
GPCRDB: ADRB2_HUMAN >>
CRYSTALLIZATION CONDITIONS:
| LCP 50 nL | Ppt solution 0.8 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid 1: 90 %w/w Monoolein | Protein: 50 mg/mL β2AR-T4L | Buffer: 100 mM Bis tris propane pH 7 |
| Lipid 2: 10 %w/w Cholesterol | Ligand: 2 mM Ligand | Precipitant: 28 %v/v PEG 400 |
| Buffer: 20 mM Hepes pH 7.5 | Salt: 0.3 M K Formate | |
| Detergent 1: 0.05 %w/v DDM | Ligand: 2 mM Ligand | |
| Detergent 2: 0.01%w/v CHS | Salt: 150 mM NaCl |
Temperature (°C): 20
Crystal growth time (d): 3 – 7 Crystal size (µm): 30 x 10 x 4
References: M. A. Hanson, V. Cherezov, M. T. Griffith, C. B. Roth, V. P. Jaakola, E. Y. Chien, J. Velasquez, P. Kuhn and R. C. Stevens. 2008. A Specific Cholesterol Binding Site Is Established by the 2.8 A Structure of the Human beta(2)-Adrenergic Receptor. Structure 16: 897-905 >>
D. Wacker, G. Fenalti, M. A. Brown, V. Katritch, R. Abagyan, V. Cherezov and R. C. Stevens. 2010. Conserved Binding Mode of Human beta(2) Adrenergic Receptor Inverse Agonists and Antagonist Revealed by X-ray Crystallography. J Am Chem Soc >>
SPACE GROUP: P 21 21 21 UNIT CELL: 40.00 75.70 172.73 90.0 90.0 90.0
CRYSTAL PACKING:
