Structures from LCP >> CXCR4 Chemokine Receptor bound to IT1t
NAME:CXCR4 Chemokine Receptor
PDB IDs: 3OE6
SOURCE ORGANISM: Homo sapiens
SEQUENCE: UniProt P61073>>
ORIENTATION IN MEMBRANE: OPM >>
GPCRDB: CXCR4_HUMAN >>
CRYSTALLIZATION CONDITIONS:
| LCP 50 nL | Ppt solution 0.8 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid 1: 90 %w/w Monoolein | Protein: 60 mg/mL CXCR4-T4L | Buffer: 100 mM Na Citrate pH 5.5 |
| Lipid 2: 10 %w/w Cholesterol | Ligand: 2 mM IT1t | Precipitant: 24 %v/v PEG 400 |
| Buffer: 25 mM Hepes pH 7.5 | Salt: 0.3 M Na Malonate | |
| Detergent 1: 0.05 %w/v DDM | Ligand: 2 mM IT1t | |
| Detergent 2: 0.01%w/v CHS | Additive: 5 mM Ni Chloride | Salt: 500 mM NaCl |
Temperature (°C): 20 Crystal growth time (d): 7 Crystal size (µm): 60 x 10 x 5
References: B. Wu, E. Y. Chien, C. D. Mol, G. Fenalti, W. Liu, V. Katritch, R. Abagyan, A. Brooun, P. Wells, F. C. Bi, D. J. Hamel, P. Kuhn, T. M. Handel, V. Cherezov and R. C. Stevens (2010) Structures of the CXCR4 Chemokine GPCR with Small-Molecule and Cyclic Peptide Antagonists Science >>
SPACE GROUP: I 2 2 2 UNIT CELL: 71.12 78.71 240.59 90.0 90.0 90.0
CRYSTAL PACKING:
