Structures from LCP >> CXCR4 Chemokine Receptor bound to IT1t

NAME:CXCR4 Chemokine Receptor

PDB IDs: 3OE9

SOURCE ORGANISM: Homo sapiens

SEQUENCE: UniProt P61073>>

ORIENTATION IN MEMBRANE: OPM >>

GPCRDB: CXCR4_HUMAN >>

CRYSTALLIZATION CONDITIONS:

LCP 50 nL

Ppt solution 0.8 µL

Lipid 60 %w/w	Protein Solution 40 %w/w
Lipid 1: 90 %w/w Monoolein	Protein: 60 mg/mL CXCR4-T4L	Buffer: 100 mM MES pH 6.0
Lipid 2: 10 %w/w Cholesterol	Ligand: 2 mM IT1t	Precipitant: 30 %v/v PEG 400
	Buffer: 25 mM Hepes pH 7.5	Salt: 0.3 M Na Malonate
	Detergent 1: 0.05 %w/v DDM	Ligand: 2 mM IT1t
	Detergent 2: 0.01%w/v CHS	Additive: 5 mM Hexamine Co Chloride
	Salt: 500 mM NaCl

Temperature (°C): 20                Crystal growth time (d): 10                Crystal size (µm): 60 x 40 x 15

References: B. Wu, E. Y. Chien, C. D. Mol, G. Fenalti, W. Liu, V. Katritch, R. Abagyan, A. Brooun, P. Wells, F. C. Bi, D. J. Hamel, P. Kuhn, T. M. Handel, V. Cherezov and R. C. Stevens (2010) Structures of the CXCR4 Chemokine GPCR with Small-Molecule and Cyclic Peptide Antagonists Science >>

SPACE GROUP: P 1             UNIT CELL: 72.50 72.74 84.27 64.7 73.9 61.3

CRYSTAL PACKING:

CONTACT US: USC | Cherezov Lab | cherezov@usc.edu