NAME:Outer membrane porin OmpF
PDB IDs: 3POQ
SOURCE ORGANISM: Escherichia coli
SEQUENCE: UniProt P02931>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP 100 nL | Ppt solution 1 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid: 100 %w/w Monoolein | Protein: 10-55 mg/mL OmpF | Precipitant: 2.0-2.1 M KSCN |
| Buffer: 20 mM Tris HCl pH 8.0 | Buffer: 100 mM Na acetate pH 4.6 | |
| Detergent: 0.05 %w/v DDM | Salt: 2.6-2.7 M LiNO3 | |
| Salt: 100 mM NaCl |
Temperature (°C): 20 Crystal growth time (d): 21 Crystal size (µm): 200 x 200 x 10
Comments:
Reference: Efremov RG, Sazanov LA (2012) Structure of Escherichia coli OmpF porin from lipidic mesophase J Struct Biol 178: 311-318 >>
SPACE GROUP: H 3 2 UNIT CELL: 77.31 77.31 330.36 90.0 90.0 120.0
CRYSTAL PACKING:
