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Outer membrane proteins >> OmpF

NAME:Outer membrane porin OmpF

PDB IDs: 3POU

SOURCE ORGANISM: Escherichia coli

SEQUENCE: UniProt P02931>>

ORIENTATION IN MEMBRANE: OPM >>

CRYSTALLIZATION CONDITIONS:

LCP 100 nL Ppt solution 1 µL
Lipid 60 %w/w Protein Solution 40 %w/w
Lipid: 100 %w/w Monoolein Protein: 10-55 mg/mL OmpF Precipitant: 1.0 M KSCN
Buffer: 20 mM Tris HCl pH 8.0 Buffer: 100 mM Na citrate pH 5.6
Detergent: 0.05 %w/v DDM Salt: 0.7 M Na tartrate
Salt: 100 mM NaCl

Temperature (°C): 20           Crystal growth time (d): 21           Crystal size (µm):

Comments:

Reference: Efremov RG, Sazanov LA (2012) Structure of Escherichia coli OmpF porin from lipidic mesophase J Struct Biol 178: 311-318 >>

SPACE GROUP: H 3 2             UNIT CELL: 85.71 85.71 332.94       90.0 90.0 120.0

CRYSTAL PACKING:

CONTACT US: USC | Cherezov Lab | cherezov@usc.edu