Structures from LCP >> ba3 cytochrome c oxidase
NAME:ba3 cytochrome c oxidase
PDB IDs: 3S8F, 3S8G, 4ESL, 4EV3, 4F05, 4FA7, 4FAA, 4GP8, 4GP5, 4GP4, 4G7S, 4G7Q, 4G72, 4G71, 4G70,
SOURCE ORGANISM: Thermus thermophilus
SEQUENCE: UniProt Q5SJ79>> , UniProt Q5SJ80>> , UniProt P82543>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP 50 nL | Ppt solution 0.8 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid: 100 %w/w Monoolein | Protein: 15 mg/mL ba3 | Buffer: 100 mM Na cacodylate pH 5.5-6.5 |
| Buffer: 20 mM Tris pH 7.6 | Precipitant: 40-45 %v/v PEG 400 | |
| Detergent: 1 mM DDM | Salt: 1-1.6 M NaCl |
Temperature (°C): 20
Crystal growth time (d): 14 Crystal size (µm): 60 x 50 x 25
Comments:
Reference: Tiefenbrunn, T., Liu, W., Chen, Y., Katritch, V., Stout, C.D., Fee, J.A., Cherezov, V. 2011. High resolution structure of the ba3 cytochrome c oxidase from Thermus thermophilus in a lipidic environment. PLoS ONE 6: e22348 >>
SPACE GROUP: C 2 UNIT CELL: 143.59 97.82 94.95 90.0 128.30 90.0
CRYSTAL PACKING:
