Structures from LCP >> β2-Adrenergic Receptor - Gs Protein complex
NAME:β2-Adrenergic Receptor - Gs Protein complex
PDB IDs: 3SN6
SOURCE ORGANISM: Homo sapiens
SEQUENCE: ADRB2_HUMAN>> , LYS_BPT4>> , GNAS2_BOVIN>> , GBB1_RAT>> , GBG2_BOVIN>> ,
ORIENTATION IN MEMBRANE: OPM >>
GPCRDB: ADRB2_HUMAN >>
CRYSTALLIZATION CONDITIONS:
| LCP 40 nL | Ppt solution 0.8 µL |
| Lipid 50 %w/w | Protein Solution 50 %w/w | |
| Lipid 1: 90 %w/w 7.7 MAG | Protein: 50 mg/mL T4L-β2AR-Gs-Nb35 | Buffer: 100 mM MES pH 6.5 |
| Lipid 2: 10% w/w Cholesterol | Buffer: HEPES pH 7.5 | Precipitant: 20 %w/v PEG 400 |
| Detergent: MNG-3 | Salt: 400 mM K nitrate | |
| Ligand: BI-167107 | Ligand: 0.01 mM BI-167107 | |
| Additive: TCEP | Additive: 1 mM TCEP | |
| Additive: 10 mM Foscarnet |
Temperature (°C): 20 Crystal growth time (d): 3-4 Crystal size (µm): 250
References: S.G.F. Rasmussen, B.T. DeVree, Y. Zou, A.C. Kruse, K.Y. Chung, T.S. Kobilka, F.S. Thian, P.S. Chae, E. Pardon, D. Calinski, J.M. Mathiesen, S.T.A. Shah, J.A. Lyons, M. Caffrey, S.H. Gellman, J. Steyaert, G. Skiniotis, W.I. Weis, R.K. Sunahara and B.K. Kobilka (2011) Crystal structure of the b2 adrenergic receptor - Gs protein complex Nature Published online 19 July 2011 >>
SPACE GROUP: P 21 UNIT CELL: 119.34 64.56 131.24 90 91.67 90
CRYSTAL PACKING:
