Channelrhodopsin

NAME:Channelrhodopsin

PDB IDs: 3UG9

SOURCE ORGANISM: Chlamydomonas reinhardtii

SEQUENCE: UniProt Q93WP2>> , UniProt Q8RUT8>>

ORIENTATION IN MEMBRANE: OPM >>

CRYSTALLIZATION CONDITIONS:

LCP 100 nL

Ppt solution 1 µL

Lipid 60 %w/w	Protein Solution 40 %w/w
Lipid: 100 %w/w Monoolein	Protein: 10 mg/mL ChR	Precipitant: 30-34 %v/v PEG500DME
	Buffer: 50 mM Tris HCl pH 8.0	Buffer: 100 mM Na citrate pH 6.0
	Detergent: 0.05 %w/v DDM	Salt: 100 mM (NH4)2SO4
	Detergent: 0.01 %w/v CHS	Salt: 100 mM NaCl
	Salt: 150 mM NaCl	Salt: 100 mM MgCl2
	Additive: 5 %v/v glycerol

Temperature (°C): 20

Crystal growth time (d): 14 - 21 Crystal size (µm):

Comments:

Reference: H.E. Kato, F. Zhang, O. Yizhar, C. Ramakrishnan, T. Nishizawa, K. Hirata, J. Ito, Y. Aita, T. Tsukazaki, S. Hayashi, P. Hegermann, A.D. Maturana, R. Ishitani, K. Deisseroth, O. Nureki (2012) Crystal structure of the channelrhodopsin light-gated cation channel Nature 482: 369-374 >>

SPACE GROUP: C 2 2 21 UNIT CELL: 59.47 139.11 90.05 90.0 90.0 90.0

CRYSTAL PACKING: