Structures from LCP >> Na+-Ca2+ exchanger
NAME:Na+-Ca2+ exchanger
PDB IDs: 3V5U
SOURCE ORGANISM: Methanocaldococcus jannaschii
SEQUENCE: UniProt Q57556>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP 35 nL | Ppt solution 4 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid: 100 %w/w Monoolein | Protein: 40 mg/mL NCX | Buffer: 100 mM MES pH 6.5 |
| Buffer: 20 mM Hepes pH 7.1 | Precipitant: 42 %v/v PEG 400 | |
| Detergent: 0.5 mM DM | Salt: 0.1-0.2 M Na Acetate | |
| Salt: 50 mM NaCl, 12 mM KCl, 10 mM CaCl2 |
Temperature (°C): Room Temperature
Crystal growth time (d): ? Crystal size (µm): 25 x 25 x 120
Comments:
Reference: Liao, J., Li, H., Zeng, W., Sauer, D.B., Belmares, R., Jiang, Y. 2012. Structural insight into the ion-exchange mechanism of the sodium/calcium exchanger. Science 335: 686-690 >>
SPACE GROUP: P 21 21 21 UNIT CELL: 49.49 72.88 96.21 90.0 90.0 90.0
CRYSTAL PACKING:
