Outer membrane proteins >> Intimin
NAME:Intimin
PDB IDs: 4E1S
SOURCE ORGANISM: E. coli
SEQUENCE: UniProt P43261>>
ORIENTATION IN MEMBRANE: OPM >>
CRYSTALLIZATION CONDITIONS:
| LCP 100 nL | Ppt solution 750 nL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid: 100 %w/w Monoolein | Protein: 20 mg/mL Intimin | Buffer: 100 mM Na citrate pH 4.5-5.5 |
| Buffer: 50 mM Tris pH 7.5 | Precipitant: 30-34 %v/v PEG400 | |
| Detergent: 0.05 %w/v LDAO | Salt: 0.1-0.15 M Mg chloride | |
| Salt: 0.2 M Na chloride | Salt: 0.05-0.1 M Na chloride |
Temperature (°C): 20 Crystal growth time (d): 7-10 Crystal size (µm): 80 x 40 x 10
Comments:SeMet phasing
Reference: J.W. Fairman, N. Dautin, D. Wojtowicz, W. Liu, N. Noinaj, T.J. Barnard, E. Udho, T.M. Przytycka, V. Cherezov and S.K. Buchanan (2012) Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enterophathogenic Y. pseudotuberculosis. Structure : in press >>
SPACE GROUP: C 2 2 21 UNIT CELL: 116.57 120.25 39.09 90.0 90.0 90.0
CRYSTAL PACKING:
