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Outer membrane proteins >> Intimin

NAME:Intimin

PDB IDs: 4E1S

SOURCE ORGANISM: E. coli

SEQUENCE: UniProt P43261>>

ORIENTATION IN MEMBRANE: OPM >>

CRYSTALLIZATION CONDITIONS:

LCP 100 nL Ppt solution 750 nL
Lipid 60 %w/w Protein Solution 40 %w/w
Lipid: 100 %w/w Monoolein Protein: 20 mg/mL Intimin Buffer: 100 mM Na citrate pH 4.5-5.5
Buffer: 50 mM Tris pH 7.5 Precipitant: 30-34 %v/v PEG400
Detergent: 0.05 %w/v LDAO Salt: 0.1-0.15 M Mg chloride
Salt: 0.2 M Na chloride Salt: 0.05-0.1 M Na chloride

Temperature (°C): 20            Crystal growth time (d): 7-10            Crystal size (µm): 80 x 40 x 10

Comments:SeMet phasing

Reference: J.W. Fairman, N. Dautin, D. Wojtowicz, W. Liu, N. Noinaj, T.J. Barnard, E. Udho, T.M. Przytycka, V. Cherezov and S.K. Buchanan (2012) Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enterophathogenic Y. pseudotuberculosis. Structure : in press >>

SPACE GROUP: C 2 2 21            UNIT CELL: 116.57 120.25 39.09       90.0 90.0 90.0

CRYSTAL PACKING:

CONTACT US: USC | Cherezov Lab | cherezov@usc.edu