Outer membrane proteins >> Invasin

NAME:Invasin

PDB IDs: 4E1T

SOURCE ORGANISM: Yersinia pseudotuberculosis

SEQUENCE: UniProt P11922>>

ORIENTATION IN MEMBRANE: OPM >>

CRYSTALLIZATION CONDITIONS:

LCP 100 nL

Ppt solution 750 nL

Lipid 60 %w/w	Protein Solution 40 %w/w
Lipid: 100 %w/w Monoolein	Protein: 20 mg/mL Invasin	Buffer: 50 mM Na citrate pH 3.8-4.4
	Buffer: 50 mM Tris pH 7.5	Precipitant: 23-35 %v/v PEG400
	Detergent: 0.05 %w/v LDAO	Salt: 0.2 M Li sulfate
	Salt: 0.2 M Na chloride

Temperature (°C): 20            Crystal growth time (d):            Crystal size (µm):

Comments:

Reference: J.W. Fairman, N. Dautin, D. Wojtowicz, W. Liu, N. Noinaj, T.J. Barnard, E. Udho, T.M. Przytycka, V. Cherezov and S.K. Buchanan (2012) Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enterophathogenic Y. pseudotuberculosis. Structure : in press >>

SPACE GROUP: C 2 2 21            UNIT CELL: 79.4 124.89 65.07       90.0 90.0 90.0

CRYSTAL PACKING:

CONTACT US: USC | Cherezov Lab | cherezov@usc.edu