G protein-coupled receptors >> Adenosine A2A Receptor
NAME:Adenosine A2A Receptor
SOURCE ORGANISM: Homo sapiens
SEQUENCE: UniProt P29274>>
ORIENTATION IN MEMBRANE: OPM >>
GPCRDB: AA2AR_HUMAN >>
CRYSTALLIZATION CONDITIONS:
| LCP 50 nL | Ppt solution 0.8 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid 1: 90 %w/w Monoolein | Protein: 70 mg/mL A2AR-T4L | Buffer: 100 mM Na citrate pH 6.5 |
| Lipid 2: 10 %w/w Cholesterol | Ligand: 200 µM ZM241385 | Precipitant: 30 %v/v PEG 400 |
| Buffer: 25 mM Hepes pH 7.5 | Salt: 0.19 M Li sulfate | |
| Detergent 1: 0.05 %w/v DDM | Ligand: 200 µM ZM241385 | |
| Detergent 2: 0.01%w/v CHS | Salt: 800 mM NaCl |
Temperature (°C): 20
Crystal growth time (d): 10 – 14 Crystal size (µm): 100 x 10 x 5
Reference: V. P. Jaakola, M. T. Griffith, M. A. Hanson, V. Cherezov, E. Y. Chien, J. R. Lane, A. P. Ijzerman and R. C. Stevens. 2008. The 2.6 Angstrom Crystal Structure of a Human A2A Adenosine Receptor Bound to an Antagonist. Science 322: 1211-1217 >>
SPACE GROUP: P 21 UNIT CELL: 47.74 76.93 86.55 90.0 101.32 90.0
CRYSTAL PACKING:
