Structures from LCP >> G protein-coupled receptors >> Sphingosine 1-Phosphate Receptor 1
NAME:Sphingosine 1-Phosphate Receptor 1
SOURCE ORGANISM: Homo sapiens
SEQUENCE: S1PR1_HUMAN>> , LYS_BPT4>>
ORIENTATION IN MEMBRANE: OPM >>
GPCRDB: S1PR1_HUMAN >>
CRYSTALLIZATION CONDITIONS:
| LCP 25 nL | Ppt solution 0.8 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid 1: 90 %w/w monoolein | Protein: 100 mg/mL S1P1 | Buffer: 100 mM Tricine pH 7.75 |
| Lipid 2: 10% w/w Cholesterol | Buffer: 25 mM HEPES pH 7.5 | Precipitant: 34-36 %v/v PEG 400 |
| Detergent1: 0.05% DDM | Salt: 80 mM Na citrate | |
| Detergent2: 0.001% CHS | Additive: 4 % glycerol | |
| Ligand: 250 μM ML056 | ||
| Salt: 500mM NaCl |
Temperature (°C): 14 (nucl.) 17 (growth) Crystal growth time (d): 3 Crystal size (µm): 50x10x5
References: Hanson, M.A., Roth, C.B., Jo, E., Griffith, M.T., Scott, F.L., Reinhart, G., Desale, H., Clemons, B., Cahalan S.M., Schuerer, S.C., Sanna, M.G., Han, G.W., Kuhn, P., Rosen, H., Stevens, R.C. (2012) Crystal structure of a lipid G protein-coupled receptor Science 335: 851-855 >>
SPACE GROUP: P 21 21 2 UNIT CELL: 107.94 69.70 81.93 90 90 90
CRYSTAL PACKING:
