Structures from LCP >> G protein-coupled receptors >> μ-opioid receptor
NAME:μ-opioid receptor
PDB IDs: 4DKL
SOURCE ORGANISM: Mus musculus
SEQUENCE: OPRM_MOUSE>> , LYS_BPT4>>
ORIENTATION IN MEMBRANE: OPM >>
GPCRDB: OPRM_MOUSE >>
CRYSTALLIZATION CONDITIONS:
| LCP 80 nL | Ppt solution 0.7 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid 1: 90 %w/w monoolein | Protein: 30 mg/mL μ-OR | Buffer: 100 mM Hepes pH 7.0 |
| Lipid 2: 10% w/w Cholesterol | Buffer: 20mM HEPES pH 7.5 | Precipitant: 30-38 %v/v PEG 400 |
| Detergent1: 0.01% MNG | Salt: 300 mM Li sulfate | |
| Detergent2: 0.001% CHS | Additive: 7.5 %v/v DMSO | |
| Ligand: 10 μM β-FNA | ||
| Salt: 100mM NaCl |
Temperature (°C): 20 Crystal growth time (d): 5 Crystal size (µm): 20x20x20
References: Manglik, A., Kruse, A.C., Kobilka, T.S., Thian, F.S., Mathiesen, J.M., Sunahara, R.K., Pardo, L., Weis, W.I., Kobilka, B.K., Granier, S. (2012) Crystal structure of the μ-opioid receptor bound to a morphinan antagonist Nature : Published online 21 March 2012 >>
SPACE GROUP: C 2 UNIT CELL: 70.88 174.73 68.35 90 107.84 90
CRYSTAL PACKING:
