G protein-coupled receptors >> Adenosine A2A Receptor
NAME:Adenosine A2A Receptor
PDB IDs: 4EIY
SOURCE ORGANISM: Homo sapiens
SEQUENCE: UniProt P29274>>
ORIENTATION IN MEMBRANE: OPM >>
GPCRDB: AA2AR_HUMAN >>
CRYSTALLIZATION CONDITIONS:
| LCP 50 nL | Ppt solution 0.8 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid 1: 90 %w/w Monoolein | Protein: 60 mg/mL A2AR-BRIL | Buffer: 100 mM Na citrate pH 5.0 |
| Lipid 2: 10 %w/w Cholesterol | Ligand: 25 µM ZM241385 | Precipitant: 25-28 %v/v PEG 400 |
| Buffer: 25 mM Hepes pH 7.5 | Salt: 40-60 mM Na thiocyanate | |
| Detergent 1: 0.025 %w/v DDM | Ligand: 200 µM ZM241385 | |
| Detergent 2: 0.005%w/v CHS | Additive: 2%v/v 2,5-hexanediol | Salt: 800 mM NaCl |
Temperature (°C): 20
Crystal growth time (d): 7 Crystal size (µm): 60 x 10 x 3
Reference: Liu, W., Chun, E., Thompson, A.A., Chubukov, P., Xu, F., Katritch, V., Han, G.W., Roth, C.B., Heitman, L.H., IJzerman, A.P., Cherezov, V. and R. C. Stevens. 2012. Structural basis for allosteric regulation of GPCR by sodium ions. Science 337: 232-236 >>
SPACE GROUP: C 2 2 21 UNIT CELL: 39.44 179.52 140.31 90.0 90.0 90.0
CRYSTAL PACKING:
